FastQCFastQC Report
Thu 26 May 2016
SRR2094159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5382818
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT177250.3292884879258411No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT150170.2789802664700906No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA149230.27723396927037103No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA131900.2450389368542648No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120390.2236560849725924No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC112730.20942562055785652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101450.1884700541612219No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGC91060.16916789681538555No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT75450.14016821672217045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69280.12870581914528784No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA67860.12606779571592427No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC58440.10856766845916024No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT57790.10736012252318394No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC54950.1020840756644568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT30100.016.59421339
TCGATCG952.8225855E-416.57832341
TCTCGTA30550.016.35008638
TGCCGTC30600.016.17571445
AATCTCG31400.016.05050336
TATGCCG31400.015.978743
ATCTCCG32550.015.82933910
ATGCCGT31750.015.73154644
TCGTATG31850.015.68229940
CCCACGA33650.015.3777519
TCTCCGA34150.015.21834111
CGTATGC33300.015.20213241
GCCCACG33600.015.19975918
CACGAGA33850.015.15480921
ACGAGAC34600.015.15144622
TCCGAGC34350.014.73675313
CGAGCCC34550.014.65144615
CCACGAG34700.014.58906120
CGAGACA35700.014.55854723
TATCGCT1402.2316231E-514.465227
Produced by FastQC (version 0.11.5)