FastQCFastQC Report
Thu 26 May 2016
SRR2094158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7150237
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT220530.3084233431703033No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA202610.28336123683732445No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT184820.2584809426596629No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA161440.2257827258033545No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA153240.2143145744679512No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC108140.1512397421232331No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107120.1498132159815122No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAG89710.12546437271939379No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89620.1253385027657125No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC83580.11689123031865938No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT82720.11568847298348292No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT80070.1119823021250904No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA78930.11038794937846116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATC34800.018.87889133
GGCGAAT34750.018.71181532
CGAATCT35650.018.3656534
CTCGTAT34850.018.20670539
TATGCCG34650.018.18154143
TAAGGCG36600.017.88844729
TCTCGTA35900.017.67444438
AATCTCG36850.017.64559436
TCGTATG36300.017.4170940
CGAGACT37750.017.40370823
ATGCCGT36200.017.34077544
ATCTCCG37450.017.24272710
ACGAGAC38600.017.07875422
TGCCGTC36900.017.0727945
AGGCGAA38250.017.05832531
AAGGCGA38400.016.99145330
CGTATGC37350.016.8673341
CACGAGA38550.016.80873121
CCACGAG38900.016.715120
ATCTCGT38300.016.56702237
Produced by FastQC (version 0.11.5)