FastQCFastQC Report
Thu 26 May 2016
SRR2094157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8020806
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA463310.5776352152140322No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT278430.3471346894563963No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA258460.3222369422723851No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA250440.31223794715892644No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC238300.2971023111642396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235040.29303788172909306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204860.25541074051660145No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT193430.2411603023436797No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA181990.22689739659580346No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT167500.20883188048682388No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT163960.20441835895295307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146780.1829990651812299No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT118760.14806492015889675No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA117780.1468430978133619No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC117210.14613244604095899No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT114900.14325243622648398No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC101170.12613445581404162TruSeq Adapter, Index 3 (95% over 22bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT97020.12096041220795016No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG91750.11439000020696174No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA89890.11207103126543642No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC85130.10613646558712429No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT81240.1012865789298482No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC80970.10094995440607839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG67050.017.64908210
AATCTCG68400.017.27065536
CACGAGA69450.017.2345121
TCTCGTA68200.017.12334338
TATGCCG67950.017.11936843
CTCGTAT68650.017.0097239
CCACGAG70600.016.85806820
ACGTAGA68950.016.80518527
ATGCCGT69400.016.7952644
TGCCGTC69500.016.77109145
ATCTCGT70150.016.71150837
GCCCACG71700.016.59943618
GACGTAG69950.016.59710526
CCCACGA71500.016.58293519
TCGTATG70700.016.42073240
TCTCCGA73300.016.26698511
TCCGAGC74150.016.23221613
ACGAGAC74950.016.2099522
CTCCGAG75200.016.12523712
CGAGCCC75250.015.93543815
Produced by FastQC (version 0.11.5)