FastQCFastQC Report
Thu 26 May 2016
SRR2094156_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094156_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5820620
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT171230.2941782834131072No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA151820.2608313203748054No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT134310.2307486144087743No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA125880.21626562118812084No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC119120.2046517381309895No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106710.183330985358948No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92000.15805876349942102No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT76990.1322711326284829No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGC64530.11086447835453954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62170.10680992746477179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG39750.015.84738910
ACCGTCG1301.1090384E-515.5752858
TCTCGTA39650.015.32258538
CTCGTAT39550.015.24596839
CGAGACC41750.015.03512223
CGTATGC41250.014.94529541
CGAGGCT41750.014.87383729
TCGTATG41000.014.87128140
ATCTCGT40750.014.74331337
CACGAGA42300.014.73312621
ACGAGAC42350.014.71573122
TATGCCG41200.014.69062943
AGACCGA42600.014.68231125
GCCCACG42950.014.66680218
GATCTCG41350.014.63834436
TGCCGTC41600.014.60534345
CGCAATA1859.625728E-814.59569336
ACCGAGG42500.014.55841727
TATAACG2950.014.4900022
TCTCCGA43700.014.41495911
Produced by FastQC (version 0.11.5)