FastQCFastQC Report
Thu 26 May 2016
SRR2094155_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094155_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6326488
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA493130.7794687984866169No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA253650.4009333456413732No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT182720.28881742919610376No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT163700.2587533557322799No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT130350.20603848454308296No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC122430.1935196905455286No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT117270.18536350657742495No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA115850.18311897533038868No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA107330.16965178784817106No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC106590.16848210255041976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91720.1449777506888498No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG91180.1441241965526529No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT89450.1413896620052073No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA87740.13868674057391717No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT83190.13149475664855445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79340.12540923178863217No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT75890.11995596925181871No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT66830.10563522763340417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG27700.019.08761428
CGCTATC28200.018.830633
TATCTCG28800.018.3601736
ACGCTAC30050.017.89439227
GACGCTA30300.017.82100326
CTCGTAT29450.017.8005839
TACGCTA29700.017.72649831
TATGCCG29400.017.67863743
GCTACGC29800.017.59137329
ACGCTAT29900.017.45894832
ATGCCGT29550.017.36211844
CGTATGC29800.017.2144241
CGAGACG31250.017.06284923
TCGTATG30200.017.06051340
CACGAGA31650.016.98937621
TCTCGTA31050.016.88478738
CTACGCT31250.016.77486830
TGCCGTC30900.016.67640145
CCACGAG32300.016.64761520
TCCGAGC32600.016.63178813
Produced by FastQC (version 0.11.5)