FastQCFastQC Report
Thu 26 May 2016
SRR2094154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5074625
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT125350.2470133261078405No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA109800.21637066778333372No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC101960.200921250338695RNA PCR Primer, Index 23 (95% over 21bp)
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT86070.1696085917678646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81100.1598147646377811No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA76730.15120329088356282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73080.1440106411803828No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGC62360.122885927531591No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT57880.11405768899179743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG30100.018.9848910
CTCGTAT29350.018.70459439
TCTCGTA29300.018.66027538
CGAGACC30600.018.52854723
TGCCGTC30450.018.17864245
CACGAGA31100.018.15831821
CCCACGA31500.017.85578219
TATGCCG31250.017.64004743
CGAGCCC32100.017.59194615
TCGTATG31800.017.47577340
GCCCACG32950.017.4114218
TCCGAGC32600.017.39096813
ATGCCGT32100.017.38442244
CCACGAG32700.017.26983620
ACGAGAC33250.017.25500522
CCGAGCC33500.017.05823314
TCTCCGA33250.017.05099511
ATCTCGT32400.016.9443237
GATCTCG32600.016.90938636
CGTATGC32900.016.82325641
Produced by FastQC (version 0.11.5)