FastQCFastQC Report
Thu 26 May 2016
SRR2094153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5179356
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT101890.19672329919009235No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC90900.17550444495416032RNA PCR Primer, Index 39 (95% over 21bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA87190.16834139225031067No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85800.1656576609138279No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74610.14405265828415734No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT71560.1381638952796448No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA63200.1220228924213744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC2000.020.2474733
ATCTCCG26300.017.36475210
CTCGTAT27300.016.23596239
TATGCCG27400.016.09505743
TCTCGTA27650.015.95076438
TCGTATG28450.015.81662240
TGCCGTC27800.015.70417845
CGAGACC29050.015.56694523
CACGAGA29050.015.48979621
CATCTCG28750.015.41874136
TCTCCGA29050.015.41115811
ATCTCGT28950.015.38994937
CCACGAG29750.015.27658320
ACGAGAC29900.015.27519222
ATGCCGT28750.015.26353244
CGTATGC30150.015.000010541
CCCACGA29700.014.99870719
CGATCGA1056.0476526E-414.99812710
CGAGCCC29600.014.97279215
TCCGAGC30000.014.92313713
Produced by FastQC (version 0.11.5)