FastQCFastQC Report
Thu 26 May 2016
SRR2094152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8388253
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT373540.44531322553098956No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA336800.4015138789924434No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT281450.3355287447815415No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA266470.31767043745580875No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC234540.2796053004123743RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196560.2343276961245685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165530.19733548809269344No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT157480.18773873415596787No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC111440.13285245449797473No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110460.1316841540187212No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC110000.13113576807947971No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG106370.1268082877328569No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC105890.12623605892669187No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT105550.12583073018899166No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC101740.12128866404005696RNA PCR Primer, Index 27 (95% over 24bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG91210.10873539460481223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG68950.016.44673310
TATCTCG67650.016.42999336
CTCGTAT68450.016.10600739
TATGCCG68250.016.05392543
ACGGACT69050.016.03198827
CGGACTC69700.015.91390728
TCTCGTA69000.015.91269638
ATGCCGT69550.015.78629344
CGAGACG72250.015.72695523
CCACGAG72250.015.63306220
TGCCGTC70400.015.6276545
CCCACGA72300.015.5901119
GACGGAC71400.015.53602326
CACGAGA72350.015.51843721
TCGTATG70850.015.49672940
ACGAGAC73550.015.47957322
ATCTCGT72300.015.27993237
CGTATGC72200.015.14464841
GCCCACG74600.015.10953818
CGAGCCC75650.015.01878415
Produced by FastQC (version 0.11.5)