FastQCFastQC Report
Thu 26 May 2016
SRR2094150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7736404
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT257570.3329324580257184No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA231730.2995319272364783No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA219700.2839820671205899No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC179130.231541682673242No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145090.18754191223726166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123300.1593763717613506No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA114140.14753624552182126No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT107770.1393024459425852No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT101180.13078427651916832No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA91680.11850466961135948No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85480.1104906103662632No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT79750.10308406851555323No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC79730.10305821671153677No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC78860.10193366323682167RNA PCR Primer, Index 46 (95% over 22bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT77930.10073155435005722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGT2200.017.38655523
GGCGAAT50550.017.09129332
GTACCGT4900.016.5316496
TATGCCG50950.016.4269943
GCGAATC52950.016.31661633
TAAGGCG54300.016.11781729
CGAGACT54400.015.96525123
CTCGTAT52850.015.87951239
TCTCGTA53350.015.85751538
AAGGCGA54800.015.84758930
ATGCCGT53050.015.6493944
AACGTCG2453.6379788E-1215.61241722
CGAATCT55500.015.60747334
ATCTCGT55500.015.44601737
AATCTCG55750.015.37635436
ATCTCCG56800.015.36919310
CGAGCCC57550.015.32428915
TCGTATG54650.015.31522140
CCCACGA56850.015.31520819
GCCCACG57650.015.29790618
Produced by FastQC (version 0.11.5)