FastQCFastQC Report
Thu 26 May 2016
SRR2094148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4118263
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA84420.20498933652367515No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT66630.16179151258673863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62360.15142306355859253No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA59120.14355566897985872No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC56380.1369023785027814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55410.1345470165455679No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT44240.10742393091456276No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA42610.10346595154316274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA17050.016.89215738
CTCGTAT16900.016.64165339
AATCTCG17050.016.62821636
CGAGCCC17550.016.15251715
TATGCCG17450.016.1171343
TGCCGTC17500.016.07381445
CGTGCGC3800.015.98533310
ATCTCCG17650.015.93333910
TCGTATG17900.015.83764740
TCCGAGC18000.015.74851313
ATGCCGT17950.015.54548344
TACCTGG27450.015.4083812
CGTATGC18700.015.40073941
CACGAGA18150.015.2474221
GCCCACG19450.015.03768718
ACGAGAC18500.014.83733822
CCGAGCC19750.014.808907514
CCCACGA19200.014.76476819
ACCTGGG27550.014.6991573
ATCTCGT19700.014.50564437
Produced by FastQC (version 0.11.5)