FastQCFastQC Report
Thu 26 May 2016
SRR2094147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5430052
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA123120.22673816015021586No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC117540.21646201546504526No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT112990.20808272185975385No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111800.20589121430144683No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA101870.18760409660901958No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94250.17357108182389414No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA65980.12150896529167676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64460.11870972874661237No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT63830.11754951886280278No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA59320.10924388937711829No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC56900.10478721014089737TruSeq Adapter, Index 10 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG1951.1310476E-814.998619
ATCTCCG35200.014.70034110
CTCGTAT34000.014.69076239
TCGATCG1859.63646E-814.59431741
TCTCGTA34450.014.49939838
TCGTATG33950.014.44717640
CGAGACC36150.014.25218623
GACCGAG36350.014.23579426
GAGACCG36750.014.20315724
TATGCCG34400.014.12828843
CCACGAG36450.014.07341820
CACGAGA36500.014.05413921
AGACCGA36600.014.01548225
TACCGTC2103.000423E-813.927287
ACGAGAC37750.013.88676922
ATGCCGT35050.013.86832444
GCCCACG36950.013.82183518
CCCACGA37300.013.81277519
ACCGAGG37150.013.7475527
TGCCGTC35200.013.74529545
Produced by FastQC (version 0.11.5)