FastQCFastQC Report
Thu 26 May 2016
SRR2094146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5361614
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA1263762.3570514401074005No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA680161.2685732318663747No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT483890.9025080880496059No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT457960.8541457852057235No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA414010.772174199783871No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT385700.719372935090068No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT346000.6453280672573595No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA316670.5906243903421619No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT278640.5196942562444816No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC275400.5136512997765225No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA250360.4669489448513078No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG227410.42414466986993093No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA206040.3842872687216946No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT205750.38374638681561185No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT195160.3639948716934863No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT188610.3517784010560999No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT184780.34463502967576554No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC175540.3274014130819563No Hit
GCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTT173970.3244731903490255No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT165130.3079856177636063No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA140470.2619920046463621No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT136730.2550164931679155No Hit
GGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGC121940.22743151595769484No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC119890.22360804041469604No Hit
GCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACC116890.21801271035177097No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC111200.20740023433242302No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA110090.20532996220914077No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC99540.18565305148785421No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA99510.18559709818722497No Hit
GTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGG96530.18003907032471939No Hit
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAAC96060.17916246861486113No Hit
CTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCG94250.1757866194768963No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA93740.17483541336619907No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT89220.16640511607139194No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG86670.16164908551790563No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG85550.15956016229441358No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGC84920.15838514298119932No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC83720.1561470109560293No Hit
TATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGT82930.154673574039459No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC78400.14622462564444214No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT77940.14536667503479364No Hit
ACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGC77380.14432221342304763No Hit
ACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCA76700.1430539386087846No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG76460.14260631220375058No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG72720.13563080072530398No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC69350.12934537995461814No Hit
GGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATG67200.12533539340952182No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA65940.12298535478309329No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC65300.12179168436966929No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC64430.120169038651421No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG60870.11352924697674992No Hit
CATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACC60480.11280185406856964No Hit
GCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGT57840.10787796361319557No Hit
GTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACA57510.10726247730627383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC16850.019.62965833
CGACTAG1151.5980368E-719.56524741
GTACCCG3200.019.0245041
CGCTACG17450.018.95312128
TATGCCG17650.018.48427443
TCGACTC1354.2726242E-818.3364444
TACGCTA18000.018.24985731
CTCGTAT18300.018.1962439
TCTCGTA18350.017.90260138
GACGCTA18600.017.90258426
ACGCTAT18450.017.80540332
GAGACGC19400.017.74371124
TATCTCG18550.017.7095836
CGAGACG19200.017.57683823
AGACGCT19000.017.52519825
ACGCTAC18900.017.49920827
GCTACGC19000.017.40694629
ACGAGAC19400.017.39579622
CACGAGA19400.017.27982321
CCACGAG19350.017.20804220
Produced by FastQC (version 0.11.5)