FastQCFastQC Report
Thu 26 May 2016
SRR2094145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4812186
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT135440.28145213007144776No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA121090.2516320025867662No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA105040.21827917707254044No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC100340.20851230604968302RNA PCR Primer, Index 23 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88150.18318078312018696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75520.15693491481833827No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT61600.1280083521293649No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA55000.11429317154407581No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51150.1062926495359905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT31350.017.87111939
TCTCGTA32000.017.5090238
ATCTCGT32600.017.25597237
GATCTCG33050.017.15718736
CGAGACC33450.017.08407423
TATGCCG32550.017.07384743
ACGAGAC33850.016.88219522
TCGTATG33300.016.75686840
TGCCGTC32750.016.69667645
CACGAGA34300.016.66070721
CCCACGA35000.016.51982319
ATGCCGT33250.016.51327744
ATCTCCG35650.016.34483510
CCACGAG35800.016.15100120
GCCCACG35850.016.06538418
CGTATGC34950.016.03081741
TCCGAGC36350.015.78250913
CGAGCCC36500.015.717648515
TCTCCGA37150.015.62432211
CTCCGAG39000.015.05622912
Produced by FastQC (version 0.11.5)