FastQCFastQC Report
Thu 26 May 2016
SRR2094144_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094144_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7208639
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT356880.4950726482488581No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA323890.44930811488826117No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT266460.3696398168919265No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA246090.3413820556141041No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC187250.2597577711964769RNA PCR Primer, Index 39 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183950.2551799306360049No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT157880.2190149902082765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153860.21343834807097428No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA118150.16390056430901867No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC115450.16015505839590524No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT109470.15185945641056517No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC107630.14930696349199898No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC105660.14657413139983844No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG104220.14457652824617795No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102160.14171884595691364No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG92410.1281934079373374No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC80100.11111667542236475RNA PCR Primer, Index 39 (95% over 24bp)
GGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGC78380.10873064943327027No Hit
GCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTT76710.10641398466478902No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG73710.10225231142799633No Hit
GCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACC73700.1022384391838737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG1302.7814167E-819.03663618
TATGCCG52850.017.7524843
TCGTATG54550.017.44683340
CTCGTAT54350.017.22125639
TATACCG3800.017.1692895
TCTCGTA54800.017.08114238
TGCCGTC55150.016.89125845
CACGAGA56650.016.79938521
ATCTCCG56900.016.76453410
CGAGACC57200.016.71675123
ATGCCGT55900.016.70488244
CATCTCG57300.016.6501636
CGTATGC56350.016.61037641
CCACGAG57500.016.55093220
TCCGAGC58400.016.48803113
CCCACGA58250.016.45335819
ATCTCGT58550.016.17928737
CTCCGAG59900.016.03758212
GCCCACG59650.016.02947818
ACGAGAC59850.015.97635622
Produced by FastQC (version 0.11.5)