FastQCFastQC Report
Thu 26 May 2016
SRR2094143_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094143_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5618942
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT703121.251338775164435No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA615731.0958112755034668No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT419230.7461013123111077No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA382120.6800568505601232No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT282310.5024255455920349No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC262540.4672409859364983No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA241070.4290309456833689No Hit
GCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTT230220.4097212606928493No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAG222670.39628456745059837No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT193930.3451361484065862No Hit
GGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGC191970.3416479472470084No Hit
GCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACC178850.3182983558114677No Hit
GTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGG158220.28158325891244296No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG153410.27302292851572413No Hit
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAAC148390.2640888622804791No Hit
CTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCG139850.2488902715137476No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC139670.2485699265092966No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGC136960.24374695449783965No Hit
TATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGT126040.224312690894478No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC123760.22025498750476513No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA122490.2179947755289163No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG114720.20416654950344745No Hit
ACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCA114110.20308093587725234No Hit
ACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGC114040.20295635726441025No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG108810.1936485551906391No Hit
GGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATG107090.19058748070366271No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC99290.1767058638441187No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT94470.16812773650270815No Hit
CATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACC94440.168074345668633No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG91310.16250390198012366No Hit
GTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACA88720.15789449330496738No Hit
GCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGT86540.15401475936217174No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC85390.1519681107226236No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT83920.14935195985294028No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC82030.1459883373062046No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA79470.14143231946512352No Hit
TACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACT78270.13929668610211673No Hit
TGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACG78010.13883396554013194No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG78000.13881616859544021No Hit
GTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACT75130.1337084454689157No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT71250.1268032309285271No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC69820.12425826783761071No Hit
GCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGT65480.11653439384140289No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64410.11463012075938851No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC60560.10777829705307512No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT60180.10710201315478962No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTT59780.10639013536712072No Hit
CATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAA56790.1010688489042955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA24000.017.34358238
TATGCCG24150.017.14208243
CTCGTAT24350.017.00173639
CGAGACT25300.016.45270323
ATGCCGT25500.016.23412544
TGCCGTC25400.016.20946345
ATCTCGT26050.016.06553337
GATCTCG26500.016.04701436
ACGAGAC26950.015.94632522
TCGTATG26250.015.85656540
CACGAGA26850.015.83783521
CCCACGA26900.015.80727219
TACCTGG59400.015.7961452
GCCCACG26550.015.76157718
CGAGCCC26750.015.72783915
CCACGAG27050.015.72031620
GTACCCG4450.015.69341851
ATCTCCG27100.015.60773210
CGGCGTT1908.021743E-915.39417641
CGTATGC27800.015.05340941
Produced by FastQC (version 0.11.5)