FastQCFastQC Report
Thu 26 May 2016
SRR2094142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5350306
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98420.18395209545024155No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA93060.17393397686038892No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT84620.1581591781853225No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT84490.15791620142847904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83620.15629012620960372No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA70530.131824235847445No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA66330.12397421754942616No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC59100.11046097176497943No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55610.1039379803697209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG38850.017.4331322
TATGCCG18100.017.27808243
CTCGTAT17750.017.23980539
ATGCCGT19000.016.57790844
CGAGACT19200.016.52301823
TCGTATG18600.016.45104240
TCTCGTA18550.016.3753238
ACCGTCG1104.6395347E-516.3649028
TGCCGTC19250.016.24573345
CCCACGA20150.015.85449219
CACGAGA20100.015.78318121
ACGAGAC20500.015.69472522
ATCTCCG20700.015.43439110
GTACCCG3950.015.396771
CGTATGC20450.015.29286741
GCCCACG20750.015.28762618
CGAGCCC20800.015.14257215
CCACGAG20850.015.10710720
CCGAGCC22400.015.06531514
TCCGAGC21500.014.96347813
Produced by FastQC (version 0.11.5)