FastQCFastQC Report
Thu 26 May 2016
SRR2094141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7794201
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT285290.3660285383966875No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA258820.3320673921547571No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT190210.24404040901690885No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA174850.22433344995850119No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162830.20891172809117958No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC157370.20190651998838624No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA151640.19455490049589433No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGC144430.18530443338579541No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141670.18176333917998777No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT127210.16321108475390872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95710.12279642262240864No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC95120.12203944958565992No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC90830.11653535750489372No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC84040.10782375255654812No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG82540.10589924483600051No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA80870.10375662624045748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATC46300.016.76470833
GGCGAAT46450.016.75900732
TAAGGCG46500.016.69238729
ATAACGC4250.016.4117813
AATCTCG47700.016.36710236
CTCGTAT46600.016.31923739
TCTCGTA47100.016.19397238
TGCCGTC46500.016.16026945
TATGCCG46850.016.13589743
ATGCCGT46600.015.9807544
CGAATCT48900.015.96535134
CGAGACT48800.015.952476523
AAGGCGA49450.015.83307530
CGTATGC48450.015.74244541
AGGCGAA49800.015.63144331
CCACGAG50550.015.6223620
TCGTATG48800.015.58343340
ATCTCCG51000.015.52932710
CACGAGA50800.015.45699821
TCCGAGC51650.015.37627413
Produced by FastQC (version 0.11.5)