FastQCFastQC Report
Thu 26 May 2016
SRR2094140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5983164
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT243100.406306763444893No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA217640.3637540271334698No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT173070.2892616682410845No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA160080.26755074739719653No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT90860.15185945095270661No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC89510.14960311968717555No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA84510.14124633722224564No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT76430.12774177675891887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74960.12528488271422944No Hit
GCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTT69110.11550744723026143No Hit
GGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGC64810.1083206143104217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61940.10352382117555194No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC61720.10315612274709501No Hit
GCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACC61180.10225359024088258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAG18200.015.20589426
TACCTGG44700.015.0991342
AGACGTA18900.014.99947625
ACCGTCG1351.5852853E-514.9984748
CGAGACG19250.014.843636523
CTCGTAT18600.014.75754939
TCTCGTA18850.014.68216738
TATGCCG19500.014.30719343
TCGTATG19900.014.13267240
AATCTCG19450.014.11379736
GTACCCG4600.013.7209751
TGCCGTC20900.013.67348645
CACGAGA21350.013.48876521
TACCCGG4350.013.4469072
ACGTAGA20450.013.42285127
ATCTCCG21300.013.41412710
ATGCCGT21350.013.38528644
TCCGAGC21750.013.3434713
ATCTCGT21350.013.27922337
ACGAGAC21600.013.22848422
Produced by FastQC (version 0.11.5)