FastQCFastQC Report
Thu 26 May 2016
SRR2094139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5536014
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA332250.6001610545060038No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA170320.30765818150026353No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT149470.2699957044906317No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA136630.24680212152642678No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC126640.2287566469304449No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT126550.22859407508723784No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT114920.20758618023726097No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94870.1713687862783584No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC85270.15402778966960706No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT83710.15120987772068495No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA79190.14304515848406452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78650.14206972742482227No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT67460.12185662825274647No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT62450.11280679564755437No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA62210.11237327073233558No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG60820.10986243893169345No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC58130.10500334717361624RNA PCR Primer, Index 33 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56360.10180610092387773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA37950.017.25385938
TATGCCG38200.017.0811143
ATCTCCG40150.016.69787410
ATGCCGT39850.016.5451944
CTCGTAT39550.016.4407339
ATCTCGT40100.016.32892237
ACCGTCG4050.016.1091828
TCGTATG40800.016.1023240
TGCCGTC40950.016.10089945
CGAGACA42050.015.99790523
CACGAGA41700.015.6470221
CCACGAG42000.015.53497520
ACGAGAC42900.015.41912522
AATCTCG42300.015.37327636
TCCGAGC43900.015.27151813
GCCCACG43400.015.24121118
GGCAATC43100.015.13985933
TCTCCGA44450.015.08255711
GCAATCT43200.015.05272834
CGTATGC43650.015.05164641
Produced by FastQC (version 0.11.5)