FastQCFastQC Report
Thu 26 May 2016
SRR2094138_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094138_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5036681
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT115680.2296750578406693No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA109280.21696827732389642No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA104630.20773600710467863No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC91200.18107162236401314No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79050.15694859372670217No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT71660.14227623309874102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66530.1320909543407653No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA64880.12881498748878478No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA56660.11249471626255465No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT53440.10610161731505331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG23750.019.32616228
TATCTCG24650.018.98640436
CGCTATC24600.018.93352933
TATGCCG25800.018.48804343
GCTACGC25250.018.17807429
GACGCTA25550.018.14093626
CTCGTAT25850.018.10358639
ACGCTAC25750.018.08741427
TCTCGTA25900.018.06989338
TATAACG2800.017.6768512
TCGTATG26500.017.65953440
CTACGCT26150.017.55174430
ATCTCGT26750.017.41159637
TACGCTA26500.017.3207931
GAGACGC27100.017.2687224
ACGCTAT26800.017.21136932
CACGAGA27500.017.18121
CCCACGA27900.016.85353519
TGCCGTC26850.016.76080745
ATGCCGT27500.016.69193644
Produced by FastQC (version 0.11.5)