FastQCFastQC Report
Thu 26 May 2016
SRR2094137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4881226
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA132690.2718374441175229No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT112950.23139678433246072No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA96810.19833132086078376No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT86730.17768077118330516No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76350.1564156218130445No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA76280.15627221521806203No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA66970.13719913808539083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63670.13043854146478773No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT50330.10310934179241035No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT50220.10288398857172358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGA654.2436412E-420.76727911
ATCTCCG14950.014.44680210
TCTCGTA15600.013.70288238
CTCGTAT15950.013.68322139
CGAGACC16450.012.99283223
GTATTAC8300.012.764741
TATGCCG16850.012.68543743
TCGTATG17050.012.66820940
GGCGTGC5700.012.6303928
ATCCGCC5550.012.56715831
GTATAAG13300.012.5421591
ACGAGAC17050.012.53560622
TACCTGG29700.012.4988252
ATGCCGT17150.012.46506644
CCCACGA17050.012.40352619
TGCCGTC17550.012.30918345
CACGAGA17500.012.21326321
GCCCACG17500.012.21313818
ATCTCGT17350.012.19105337
CGAGCCC17550.012.05014915
Produced by FastQC (version 0.11.5)