FastQCFastQC Report
Thu 26 May 2016
SRR2094136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4884705
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT202880.41533726192267495No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA178100.3646074839729318No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT146980.3008984165881051No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA129440.2649904139553975No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99750.20420885191633886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83600.17114646636797923No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT76940.15751207084153496No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC76710.15704121333836946RNA PCR Primer, Index 39 (95% over 21bp)
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC71520.14641621141911332No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT64780.13261803936982888No Hit
GCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTT59690.12219775810412296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59280.1213584034245671No Hit
GGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGC53510.10954602171471972No Hit
GCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACC51300.10502169527125998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT22650.017.0850439
TCTCGTA22850.016.93688638
CACGAGA23600.016.87378921
TATGCCG22950.016.7643643
ATCTCGT23750.016.67402337
CGAGACC23900.016.56784623
ACGAGAC24350.016.53885322
CCCACGA25000.016.10867719
TCGTATG24000.016.03026240
CGTATGC24300.016.01818741
CCACGAG25050.015.98687620
TGCCGTC24200.015.90022245
ATGCCGT24150.015.83996444
GCCCACG25350.015.7975218
ATCTCCG25400.015.76642210
TCCGAGC26000.015.57564313
TCTCCGA26250.015.34159811
CCGAGCC26600.015.30889414
CGAGCCC27000.015.08209415
CATCTCG26500.015.02860636
Produced by FastQC (version 0.11.5)