FastQCFastQC Report
Thu 26 May 2016
SRR2094135_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094135_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5208226
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA322750.6196927706286172No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA157900.303174247814899No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT116290.22328140138311972No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT106060.20363939660068514No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT101280.19446160746480662No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA90620.17399398566805666No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC81520.1565216255976603No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79060.15179832825994877No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT76540.1469598285481467No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC73840.1417757217140731No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT72660.13951007502362608No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA69690.1338075575061451No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68960.13240592862137704No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA67370.1293530657079781No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG60810.11675760614074736No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA58300.11193830682462705No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT57390.1101910708175874No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT52410.1006292737680738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT23550.017.9614539
TCTCGTA23500.017.8081838
ATCTCCG24000.017.53126110
TATGCCG24200.017.47884843
CGAGACT24250.017.35002523
ACGAGAC25000.017.09951822
TCGTATG25550.016.64320840
CCCACGA25450.016.61971919
ATGCCGT25900.016.59171944
CACGAGA25400.016.56449521
CCACGAG25550.016.46724720
TGCCGTC25450.016.4430745
CGAGCCC25500.016.41067315
GCCCACG25950.016.29949218
GATCTCG25750.016.25165736
CGTATGC26550.016.01649941
TCCGAGC26550.015.84640313
CCGAGCC26850.015.83693414
ATCTCGT26150.015.83159737
TCTCCGA27750.015.32315711
Produced by FastQC (version 0.11.5)