FastQCFastQC Report
Thu 26 May 2016
SRR2094134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5843298
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT168510.28838166391650744No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA145920.24972198919172017No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT132050.22598539386490302No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC128840.22049192082964106RNA PCR Primer, Index 27 (95% over 21bp)
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA124390.2128763585221907No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112400.1923571243499818No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96200.16463305482622997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73440.12568244850767496No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT71450.12227683749827581No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC66690.11413075287277837RNA PCR Primer, Index 27 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2151.546141E-1015.6979492
ATCTCCG41250.015.65428310
TATCTCG43300.014.96523636
CGCCCGA4700.014.84068526
TCTCGTA44250.014.79661738
CTCGTAT44050.014.65910639
TATGCCG44250.014.59260143
ACGGACT43450.014.55108827
CACGAGA45450.014.25718721
TGCCGTC44950.014.21482745
CGGACTC44550.014.191195528
TCGTATG46050.014.16890140
ATCTCGT46600.014.09895937
CGAGACG46850.014.02360423
ATGCCGT45450.013.95944544
GACGGAC45850.013.93699526
CGTATGC46700.013.87533441
GCCCACG47000.013.83392718
CGTCGAA1150.001202648213.69589137
CCACGAG47850.013.68292320
Produced by FastQC (version 0.11.5)