FastQCFastQC Report
Thu 26 May 2016
SRR2094133_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094133_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6728382
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC152010.2259235578479343No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111800.16616179045720056No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT111690.16599830390129455No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT107290.15945884166505409No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA104450.15523791603984435No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA95810.14239679019413584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92560.13756650558782185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70100.104185523354649No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC69900.10388827507118352RNA PCR Primer, Index 26 (100% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA47100.016.76736538
ATCTCCG47900.016.6282410
CTCGTAT47600.016.54359839
CCCACGA48800.016.5047719
CGAGACT47650.016.47951123
TATGCCG47850.016.45655343
TGCCGTC48150.016.21408345
CACGAGA49200.016.09693521
CCACGAG49950.016.03541420
ATGCCGT48850.016.027844
ATCTCGT49700.016.02624937
CATCTCG49550.015.98370936
TCGTATG49500.015.95392640
ACGAGAC49750.015.91957222
GCCCACG49550.015.89194918
CGTATGC50150.015.88173841
TCCGAGC51200.015.51151713
TCTCCGA52150.015.35814611
CGAGCCC51700.015.2309515
CCGAGCC54000.014.91553514
Produced by FastQC (version 0.11.5)