FastQCFastQC Report
Thu 26 May 2016
SRR2094132_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094132_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5365145
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113900.21229621939388407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96350.1795850811115077No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT94180.17554045603613697No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT92140.17173813568878382No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC78950.1471535252076132No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA78730.1467434710525065No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA78510.1463334168973998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68100.12693039983075946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG22650.017.08548410
CTCGTAT23000.016.62984339
TCTCGTA23650.016.36320538
TATGCCG23300.016.22259743
AATCTCG24050.015.90365136
CCCACGA24650.015.69865319
TGCCGTC23600.015.63503645
ACGAGAC24550.015.5806222
TCGTATG24550.015.48810340
CGTATGC24450.015.45942841
CACGAGA25350.015.17739921
TACCTGG41850.015.1611752
ATGCCGT24350.015.15346444
CGAGACA25850.015.14523423
TCTCCGA26050.015.1139211
TCCGAGC26100.014.91270613
CCACGAG26350.014.85757220
GCCCACG25900.014.85412918
CGAGCCC26000.014.53740215
CCGAGCC27750.014.107079514
Produced by FastQC (version 0.11.5)