FastQCFastQC Report
Thu 26 May 2016
SRR2094131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4544495
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT108620.2390144559516514No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT104420.22977250497580037No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA96350.21201475631505812No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA95620.2104084172168745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80630.17742345409115864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66720.14681499264494735No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC62290.13706693482994262No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT48640.10703059415842683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46090.10141940963737445No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG950.00533167614.22956851
GCGAATC22150.014.2206933
GGCGAAT22150.014.2206932
GCCCACG23700.014.14457618
TAAGGCG22650.014.1049729
TCTCGTA22750.014.04374338
CTCGTAT23300.014.001471539
AGGCGAA23400.013.84533431
CACGAGA23700.013.76546221
TATGCCG23050.013.76274243
AATCTCG23350.013.68257536
ATCTCCG23700.013.67022710
CGAATCT23350.013.58636834
TAGCGCG1000.00755903413.49905329
CGAGACT23550.013.47157823
ACGAGAC24100.013.44422222
TCGTATG24150.013.41550340
AAGGCGA23700.013.38513730
CCCACGA25150.013.32879219
CCACGAG24700.013.20815720