FastQCFastQC Report
Thu 26 May 2016
SRR2094129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4803172
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT127520.2654912212179784No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA115850.24119477711812112No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT100800.20986131664658272No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA89510.18635601639916288No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72920.15181634136774616No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC68310.14221851726317525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62270.12964349392443159No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA57780.12029550472063046No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC48980.10197427866418275No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT48610.10120395438680937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG20600.015.72635810
TCTCGTA20750.015.18135838
CCGCGAT900.003757269814.99937922
ACGAGAC21900.014.89664322
TATGCCG21200.014.85895843
TGCCGTC21100.014.50448745
CCCACGA22300.014.42735319
CTCGTAT21750.014.37856539
AATCTCG22250.014.25902536
TCTCCGA22750.014.24013111
CCACGAG22950.014.11691520
CACGAGA22900.014.04963721
ATCTCGT22700.013.97635637
ATGCCGT22200.013.78579544
CCGAGCC25000.013.67843614
TCGCACG1656.9247944E-613.63551518
TCGTATG23100.013.63551540
CGAGCCC24100.013.62914815
CGAGACA23650.013.5083923
TCCGAGC24200.013.386913
Produced by FastQC (version 0.11.5)