FastQCFastQC Report
Thu 26 May 2016
SRR2094128_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094128_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3928284
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA97260.24758902360419968No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT81560.20762246314166696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80830.20576414536219886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68450.17424911233505522No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA68430.17419819951917936No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC61030.15536045764511935No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT57520.1464252584589098No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA56050.1426831664920357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55320.14082484871256765No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA40920.10416762128196434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1608.1854523E-1018.2796942
TACGCCG850.002568104315.881000510
GAACCGT1855.638867E-915.8094646
CCTATAC6450.015.347533
ATAACGC2001.5787009E-814.6237553
GACCGCG2052.185152E-814.2670787
CGCAATT1453.1068827E-513.96610734
ACCTGGG25700.013.2187193
AACCGTG3101.6370905E-1113.0634057
CGATGCG2451.619992E-812.85768533
ATCTCCG21300.012.67497610
TAGACCG1651.0449682E-412.2716835
ACCGTGT4600.012.227228
CGGCGTT2401.7160164E-712.18770441
TCGCACT1300.00302566512.1143534
ACCGTGC3900.012.1143538
GTATAGG7100.012.0574971
TACCTGG30250.011.9741872
CTGTACG2301.3586723E-611.7381319
TCGCACG2351.7598995E-611.48867618
Produced by FastQC (version 0.11.5)