FastQCFastQC Report
Thu 26 May 2016
SRR2094127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11144
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA890.7986360373295047No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA620.5563531945441493No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT550.49353912419239054No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT360.3230437903804738No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA300.26920315865039485No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA290.26022972002871503No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC280.25125628140703515No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT270.24228284278535536No Hit
CTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCG240.21536252692031585No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT230.20638908829863603No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT220.1974156496769562No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.18844221105527637No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC190.17049533381191673No Hit
GCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTT180.1615218951902369No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA180.1615218951902369No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA180.1615218951902369No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG170.1525484565685571No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT160.14357501794687724No Hit
GCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACC160.14357501794687724No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG160.14357501794687724No Hit
TTCTATATCCTGGCTCACACTGAATTCACCCCCACTGAGACTGATACATAC150.13460157932519742No Hit
GGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATG150.13460157932519742No Hit
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAAC150.13460157932519742No Hit
ACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCA150.13460157932519742No Hit
GGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGC140.12562814070351758No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGC140.12562814070351758No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG140.12562814070351758No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT130.11665470208183776No Hit
ACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGC130.11665470208183776No Hit
TGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACG120.10768126346015792No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT120.10768126346015792No Hit
GTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACT120.10768126346015792No Hit
TCTATATCCTGGCTCACACTGAATTCACCCCCACTGAGACTGATACATACG120.10768126346015792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.10768126346015792No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC120.10768126346015792No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA120.10768126346015792No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers