FastQCFastQC Report
Thu 26 May 2016
SRR2094126_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094126_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5673983
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT139170.2452774356215026No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT126710.22331755311921098No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA110480.19471330809415538No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA101570.17901005343160173No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC93950.16558033395588248RNA PCR Primer, Index 39 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC62690.11048676035864048RNA PCR Primer, Index 39 (95% over 24bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA62520.11018714719448401RNA PCR Primer, Index 39 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61960.10920018618314506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG44200.016.6465952
GTATACG1907.879862E-915.4147821
ACCTGGG46300.014.7249543
TATACTG21450.012.90261655
TCTATAC14450.012.61274053
GCACCGT4300.012.5586446
ATCTCCG43050.012.4911310
ATTATAC15950.012.4140723
GTACATA32950.012.375731
TTGCGCG2003.6724323E-612.37407918
TATGCGA1852.248325E-512.16125812
CGATCGT1300.00302530712.114696542
CATAAGC23050.012.0069894
TATACAG28200.011.9685665
CTCGTAT44800.011.95255139
GTACCTG64050.011.8538221
GTATTAT13150.011.8214561
TCTCGTA45700.011.76670538
TGCTTGA26600.011.75695445
CATGGGG76850.011.7408754
Produced by FastQC (version 0.11.5)