FastQCFastQC Report
Thu 26 May 2016
SRR2094125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3611381
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA91200.25253497207854836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82230.2276968284431911No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT72960.2020279776628387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72680.20125265099417647No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA60840.1684674090050316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54230.15016416157697016No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT53610.14844736681064666No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC42830.11859729006715158No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA40740.11281003029035154No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA38520.10666279741738686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC1351.58406E-514.9993543
TACGACT1251.3812789E-414.4005764
ATCTCCG15200.014.21070110
GGACCGT2408.058123E-1014.0630626
CGAGACT15750.014.00017223
CTCGTAT15350.013.92525339
TCCGAGC16950.013.40613113
TACCTGG22500.013.3994242
ATGCCGT16150.013.37385244
CGGCGTT1352.6541317E-413.33349841
GTATAGG6600.013.3083341
TCTCGTA15800.013.24383438
TATGCCG16400.013.03333643
TGCCGTC16400.013.03279545
ATTATAC12400.012.8825093
TACGCTG1607.828153E-512.6567555
CCCACGA17600.012.655354519
CGTATGC17450.012.63625941
TCGTATG17100.012.63173440
GCCCACG17850.012.6041518
Produced by FastQC (version 0.11.5)