FastQCFastQC Report
Thu 26 May 2016
SRR2094124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5841010
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT1008801.7270985668574441No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA935931.6023427455183266No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA865391.4815759603219305No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT593801.0166050049563347No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA527020.9022754626340307No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA498370.8532257263726649No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT408920.7000844032110886No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC368210.6303875528376085No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT352650.6037483243480152No Hit
GCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTT349740.598766309251311No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT285380.48857988601286423No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT281150.4813379877795107No Hit
GGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGC270360.46286515517008187No Hit
GCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACC255010.43658545354313727No Hit
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAAC225110.38539567643267175No Hit
GTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGG213580.36565593964057586No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC205710.35218224245464397No Hit
CTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCG198360.3395988022619376No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT191060.32710096370319514No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC189080.3237111389982212No Hit
TATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGT182600.3126171672364882No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA181480.31069969063569486No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC169930.29092571319001337No Hit
ACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCA167820.287313324236733No Hit
ACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGC167190.28623474364878676No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG161410.27633919476254964No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGC155950.266991496333682No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT152660.26135890881885154No Hit
GGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATG152150.26048577215241886No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG151860.2599892826754277No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA149920.25666793927762493No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT149050.2551784708466515No Hit
CATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACC135130.23134697595107698No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT132560.2269470519653279No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG129850.2223074434044797No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT128960.2207837343199207No Hit
GCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGT127490.2182670462813794No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC123590.21159011883218828No Hit
GTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACA120730.2066937053694481No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC116730.19984557465232894No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA113060.19356241471937216No Hit
TGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACG110690.18950489726947908No Hit
TACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACT110650.18943641596230787No Hit
GTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACT109090.18676564498263143No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTT108140.18513921393731564No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG106630.18255404459160315No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA100890.17272697701253723No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT98240.1681900904124458No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT97940.16767648060866186No Hit
GCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGT88400.15134368884833274No Hit
GTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCA84660.14494068662782636No Hit
CATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAA83750.14338273688968176No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC81390.13934233976658147No Hit
GATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTA78040.1336070302909942No Hit
ACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCA76060.1302172055860202No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC75350.12900166238373156RNA PCR Primer, Index 27 (95% over 21bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA72840.12470446035873933No Hit
CAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTA69920.11970532493524237No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA69850.11958548264769278No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG68040.11648670349819637No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67000.1147061895117454No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT66730.11424394068833986No Hit
GTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCAT66120.11319960075397918No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA65940.11289143487170884No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGA65020.11131636480677144No Hit
ATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTAC64890.11109380055846507No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA64400.11025490454561795No Hit
TGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGT64170.10986113702938362No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC62840.10758413356594151No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG62540.10707052376215757No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACG61410.10513592683457142No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT61340.10501608454702184No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA60570.10369781938397639No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA59610.10205426801186782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG59650.017.879952
TCTCGTA20750.017.0239938
TACGCAG9650.017.0210574
TATCTCG20800.016.98277536
TATAGCG800.001711752416.8753285
CTCGTAT21300.016.68989439
AGTACGC10250.016.6835352
ACGGACT21100.016.63482327
GTACGCA10150.016.4039783
CGGACTC21400.016.2957928
TCGTATG21850.016.26950340
TATGCCG21450.016.25822343
TGCCGTC21750.016.24030545
GACGGAC21700.016.17501326
ACCTGGG44450.016.0967753
ATGCCGT21950.016.09232944
GAGTACG11400.016.0090831
TCGCACG850.002566807415.88238922
ACGCAGT10450.015.7180085
ATCTCGT22950.015.68644537
Produced by FastQC (version 0.11.5)