FastQCFastQC Report
Thu 26 May 2016
SRR2094123_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094123_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6716402
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT170070.2532159331737439No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT149940.22324452884148388No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA144870.2156958442928223No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC141640.21088672178943427No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138500.20621159960347818No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA134090.19964558404931687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118860.17696975255501382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83710.12463518413579175No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT74260.11056515080544614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41000.017.6698643
CTCGTAT41350.017.3031739
TCTCGTA42000.017.19634838
CGAGACT43450.017.19171123
TCGTATG42150.017.08138840
TGCCGTC42450.016.9074245
ATGCCGT42900.016.83495944
ATCTCCG44400.016.82374610
TCCGAGC44600.016.69711113
CACGAGA44700.016.66050121
ACGAGAC44750.016.44089722
CGAGCCC45900.016.37125415
TTAGTCG1803.921741E-916.24968339
GCCCACG46100.016.1539418
CCCACGA46250.016.1015519
TATAACG3500.016.0718332
CCACGAG46100.016.05645420
ATCTCGT46150.015.74772437
CATCTCG46800.015.72084736
TCTCCGA47850.015.65706911
Produced by FastQC (version 0.11.5)