FastQCFastQC Report
Thu 26 May 2016
SRR2094122_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094122_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5189096
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT131430.2532811110066185No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA113290.21832319155398167No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT99730.1921914722718562No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99190.19115082858362997No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84830.1634774149485768No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA82500.1589872301456747No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC74820.14418696435756825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59810.11526092406076126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG3100.015.24185424
ATCTCCG23100.014.31796810
CTCGTAT23750.014.2100439
TATGCCG23950.013.99743443
TCGTATG24400.013.92370340
TCTCGTA24600.013.81050238
AATCTCG24450.013.80307536
CGACTCG1150.001202823813.69557824
ACCGTCG1656.915474E-613.6373428
ACGAACG1000.00755742613.49953841
TCCGAGC25300.013.3388213
ACGAGAC25850.013.230103522
CACGAGA25550.013.20932221
TATATCG2053.3674587E-713.1716775
CCCACGA25950.013.09140619
ATGCCGT24950.013.0758344
CCACGAG25500.013.0582520
CGAGCCC26300.013.0027315
TGCCGTC25100.012.99768745
CGTATGC26600.012.94128641
Produced by FastQC (version 0.11.5)