FastQCFastQC Report
Thu 26 May 2016
SRR2094121_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094121_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6001952
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT173110.2884228331049632No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT161040.2683127089320274No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA152850.25466714828775705No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA137740.22949200526761962No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115490.1924207324550413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97240.16201395812562314No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC73870.12307662573775999No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT72590.12094398622314873No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA70480.11742846327328176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67830.11301323302818815No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT65900.1097976125100634No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC61930.10318309776552695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGA1257.64401E-616.1984211
CGCAATA1401.2574801E-616.07066536
ATCTCCG24700.015.66805410
TAGCGCG2302.5465852E-1115.65090729
GACCGTA24100.015.40434526
ACCGTAC24250.015.40171327
TATGCCG25600.014.85280943
CGTACTA25050.014.819159529
TCTCGTA26100.014.74116938
CGAGACC26600.014.71783423
CTCGTAT26250.014.57109939
CCGTACT25950.014.3054428
GCCCACG27950.013.92552818
TGCCGTC27400.013.87707745
CCGAGCC29000.013.80922814
CACGAGA27750.013.78335821
ATGCCGT27300.013.76308144
CGATCGT1150.001203170313.69511542
CGCGCGT2652.4556357E-1013.58414632
CGAGCCC28350.013.57033215
Produced by FastQC (version 0.11.5)