FastQCFastQC Report
Thu 26 May 2016
SRR2094120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1660894
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA40090.24137603001756885No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT38060.22915369674404268No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT37590.22632389544426074No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA33620.20242110574184746No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA30040.18086644903287025No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28730.1729791305164568No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28630.17237704513352448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23650.1423931930634947No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC22040.1326996183982843No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC18630.11216850684029203RNA PCR Primer, Index 33 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGT750.001111194117.9983396
ACCGTGT1551.0923031E-817.4177468
TCGCACG850.00256687515.88136618
CCGATCG900.00375641314.99906614
CGATCGC900.00375641314.99906615
ATCTCCG8350.014.81899210
GGCGACG1108.583481E-414.31987836
GGCCGAT950.00538064114.20921412
TGGGACG950.00538064114.2092144
ACACCGC1150.001203009913.6943886
GCCGATC1150.001203009913.69438813
ACCGCAC1352.6551317E-413.3321038
CCGTCTT6500.013.15619644
TCTCCGA10150.012.85595611
GGACAGT6050.012.643466
AATTACG1250.00225024512.60149338
CTCGTAT9300.012.58099839
GTGCGCC2156.006685E-712.5569811
ATAGCCC3601.8189894E-1212.4988463
TCTCGTA9400.012.44828338
Produced by FastQC (version 0.11.5)