FastQCFastQC Report
Thu 26 May 2016
SRR2094119_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094119_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414091
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA281541.9909609777588573No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA180451.2760847781366262No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT165001.1668273116793757No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA94580.6688395584159718No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT81760.5781806121388228No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT75230.5320025373190268No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA72950.5158791053758209No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC69090.4885824179632004No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG64110.4533654481925138No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA60520.4279781145626413No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA56710.401035011183863No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT51330.3629893691424385No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA49760.3518868304797923No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT48920.34594661871124277No Hit
TATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGT47910.33880422122762965No Hit
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAAC47250.3341369119809121No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT46340.32770168256498344No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT45460.3214786035693601No Hit
ACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCA42720.30210219851480563No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC41090.2905753590115488No Hit
GGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGC38540.2725425732855948No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT33440.2364770018336868No Hit
GTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGG30230.2137769068610153No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC29850.2110896682038143TruSeq Adapter, Index 10 (95% over 22bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT28900.20437157156081184No Hit
CTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCG28480.2014014656765371No Hit
GCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTT28270.19991641273439967No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC28200.1994213950870206No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG27890.19722917407719873No Hit
ACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGC26990.1908646614680385No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA25220.17834778667002335No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC24970.17657986650081217No Hit
CATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACC24370.17233685809470536No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT24070.17021535389165196No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG23870.168801017756283No Hit
TGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACG23020.162790089180965No Hit
GCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGT22370.15819349674101596No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21870.1546576564025936No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC21280.15048536480325522No Hit
TACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACT21050.14885887824758096No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA21020.14864672782727562No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA19910.140797162275978TruSeq Adapter, Index 10 (96% over 28bp)
CATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAA19800.14001927740152506No Hit
ACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCA19160.13549340176834446No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA18700.1322404286569959No Hit
GGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATG18410.13018964126071095No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC17880.12644165050198325No Hit
GTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACA17560.12417871268539296No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT17530.1239665622650876No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17110.12099645638081283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17070.12071358915373903No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG17020.1203600051198968RNA PCR Primer, Index 10 (95% over 24bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA16900.11951140343867545No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGA16860.11922853621160166No Hit
GTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCA16460.11639986394086378No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC16140.11413692612427347No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15180.10734811267450256No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT14660.10367083872254333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG2900.020.9609261
AGTACGC2900.019.3952262
GTACCCG600.006488567718.7613221
GTACGCA2900.018.6194173
CGCAGTG3050.017.7037076
TACGCAG3150.017.1416854
AGACCGT1053.1150703E-517.1416856
ACGCAGT3250.016.6142485
GACCGTG952.8214013E-416.5778147
TATGACC2057.4578566E-1116.462294
CCCCGTA850.00256551615.8823925
TGCTTGA6150.015.36697745
TGTACCG1108.594513E-414.3172035
CTATACT2551.3278623E-1014.1166824
TATACTG3350.014.1035145
TAGAGTG2752.910383E-1113.908145
GACTGAG3350.013.4319177
ATAGACC1851.5295318E-613.3774633
CTAAGAC2703.2923708E-1013.3324213
CGTACTC1200.001657589113.1241045
Produced by FastQC (version 0.11.5)