FastQCFastQC Report
Thu 26 May 2016
SRR2094118_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094118_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3939695
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA279460.7093442512681819No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT274550.6968813575670199No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT138980.3527684249669073No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA130440.33109162003657644No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC118950.30192692581532327RNA PCR Primer, Index 23 (95% over 21bp)
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT105490.2677618445082678No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC103110.26172076772440506No Hit
GGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGC80910.2053712279757697No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTC77610.19699494504016174RNA PCR Primer, Index 23 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72040.182856794751878No Hit
GCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTT71830.18232375856506658No Hit
GCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACC68800.17463280786964472No Hit
GTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGG63770.1618653220617332No Hit
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAAC62500.15864172226530227No Hit
CTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCG61220.15539273979330886No Hit
TATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGT60130.15262602815700202No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT59990.15227067069912772No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58750.14912321892938413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51660.13112690195560822No Hit
ACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCA51080.12965470677298624No Hit
ACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGC49150.12475585038943371No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC46680.11848632952550894No Hit
GGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATG44990.11419665735545519No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA43770.11109997093683648RNA PCR Primer, Index 23 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG43620.11071923080339975RNA PCR Primer, Index 23 (96% over 26bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG1552.0703555E-715.96733718
ATACCGT3350.015.4467816
ACCGTCG2405.0931703E-1114.9990498
ATCTCCG42050.014.92770910
TACCGTC3150.014.2848077
CGTCGTA3200.014.06160710
CCGTCTT31250.014.04089144
CGGCGTT2804.0017767E-1113.66071541
GCCGTCT32650.013.50671943
TATGCCG44700.013.48976243
TGCTTGA16300.013.3904245
TCTCGTA45500.013.30253638
CGTCTTC33250.013.26399945
CGAACGT1709.422751E-613.2344544
CTCGTAT45850.013.15158239
TCTCCGA47600.013.09265611
TCGTATG46450.013.07857940
TCGCACG3101.6370905E-1113.06418418
GTATTAG8200.012.9128941
CGAGCCC48150.012.89654215
Produced by FastQC (version 0.11.5)