FastQCFastQC Report
Thu 26 May 2016
SRR2094117_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094117_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300858
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA70290.5403356861394556No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT67920.5221169412802934No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT54960.4224903871137357No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA51990.3996593017838996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30740.2363055767808631No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA30310.2330000661102134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.19856125726251442No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT21460.16496804416777233No Hit
TATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGT20590.1582801504852951No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT20080.1543596610852222No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC20060.1542059164028664RNA PCR Primer, Index 39 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19620.15082353339103885No Hit
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAAC19530.15013168232043775No Hit
ACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCA19000.14605744823800906No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG18500.1422138311791141No Hit
GGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGC16680.12822306508473638No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC16130.12399508631995192RNA PCR Primer, Index 39 (95% over 21bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA15900.12222702247286021No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA15240.11715344795511885No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC14690.11292546919033437No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA13260.10193272440189474RNA PCR Primer, Index 39 (96% over 30bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG13110.10077963928422627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACCA651.5228728E-524.2329869
ACCGTGC754.5986293E-521.0027268
TCCGACC1002.044686E-518.0023388
CGACCAT952.8177473E-416.58046310
ATCTCCG8550.015.7915247
CCGTCTT8500.015.61787444
TATACTG4350.015.5192575
GCCGTCT8750.015.17106543
CGTTATT1056.0369505E-415.00021844
CGATGCG1056.040473E-414.99906533
ATAGCAC2304.2382453E-1014.6735633
CGTCTTC9050.014.66872145
TGCTTGA5000.014.40020945
CGTACTG950.00537188714.2123725
GAATGCG950.005378740414.209640511
GCTTACC27500.013.74512627
GCACGGG1000.00755291213.49967822
CGAGACC10450.013.34896320
TATCGTG1352.653765E-413.33250214
TCTCGTA10150.013.30017535
Produced by FastQC (version 0.11.5)