FastQCFastQC Report
Thu 26 May 2016
SRR2094116_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094116_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838225
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT41030.489486712994721No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC28260.3371409824331176TruSeq Adapter, Index 1 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22760.27152614154910676No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT20480.2443258075099168No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17780.21211488562140235No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT17020.20304810760833908No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG16470.19648662351993795RNA PCR Primer, Index 1 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC14100.16821259208446418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13820.16487220018491455No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA13720.16367920307793254TruSeq Adapter, Index 1 (96% over 28bp)
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA10770.1284857884219631No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10560.12598049449730084No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA9610.1146470219809717No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG9460.11285752632049868No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA8640.10307495004324614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC501.98192E-631.4976623
TCGCGTA300.005142336230.0049349
TAACGCA651.5211728E-524.2347534
GTACCGT654.232212E-420.7726466
GTCGCGT600.00650288318.7530848
ATCGTGT600.00650288318.7530848
GTTCGTT600.00651184918.7486139
ACGGACC750.001108658518.002968
GAGCGAA750.001110435317.99866316
GCTTACC4100.017.01093127
TATACTG3450.016.9593095
GCGAAAG850.002565658415.88117418
AGCGAAA850.002565658415.88117417
CTAAACC1351.5780797E-515.0024664
TCGTTAT900.00375315614.99978141
TGCTTGA5300.014.8591645
TATACTA2601.0913936E-1114.7139585
GACGGAC950.00536854914.2128637
ACGAATG950.00537492714.21031937
ATTACAC2254.8930815E-913.99896053
Produced by FastQC (version 0.11.5)