FastQCFastQC Report
Thu 26 May 2016
SRR2094115_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094115_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4095903
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT113170.2763004885613746No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT110440.2696352916560768No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA103480.2526427017436692No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA96560.2357477703939766No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84950.2074023725659519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74620.1821820487448067No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGC62000.15137077220822856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60280.14717145401148415No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC57910.14138518417062124RNA PCR Primer, Index 27 (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT48850.11926551971567687No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC42620.10405519857281777No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT42250.10315185686770413No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA600.006511678518.75087
ACCGTAT2453.6379788E-1215.612918
TATGCGA1951.1305019E-814.99899112
TACCTGG31200.014.7835612
ATCTCCG21450.014.68540210
CCCCGTA2509.640644E-1114.39973525
TATCTCG20950.014.39114236
ACGGACT21950.014.04302127
CGAGACG22200.013.98639923
TCTCGTA21600.013.95841738
ACGAGAC22750.013.94496722
CTCGTAT21900.013.76703839
CGGACTC22650.013.70768728
GTACCGT2804.0017767E-1113.6612966
CGCCCGA2804.0017767E-1113.66046226
ACCGTTA2652.4556357E-1013.58465635
GACGGAC22700.013.57904626
CACGAGA23450.013.52870121
TATGCCG22600.013.34046343
ACCTGGG30700.013.2652273
Produced by FastQC (version 0.11.5)