FastQCFastQC Report
Thu 26 May 2016
SRR2094114_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094114_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685737
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC28570.41663203239726015RNA PCR Primer, Index 26 (100% over 23bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT26210.38221650574491384No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT20340.2966151746223406No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA19840.28932374948413164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18770.2737200996883645No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA17010.24805428320186892RNA PCR Primer, Index 26 (100% over 29bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG16360.23857543052219732RNA PCR Primer, Index 26 (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC14350.20926390146659726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11210.16347375159864497No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10420.1519532998802748No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10350.15093250036092554No Hit
ACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCA8790.12818325392971358No Hit
TATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGT8490.12380839884678821No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8330.12147514280256133No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA8160.11899605825557028No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT7600.11082966210077624No Hit
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAAC6980.10178829492939713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG301.1395503E-437.499395
CGCCCGA352.81826E-432.1446826
CGGCGCC406.161542E-428.12659523
TTAGGAC803.3905962E-928.1245443
TACCGTA450.001226165924.9995967
ATACCGT550.003935312420.4542146
GCCGGCG600.00650574918.75106421
CGCCGGC600.00650574918.75106420
TAACACC1253.8282997E-717.9997084
CTATACT1902.3646862E-1117.7628694
TTATACC1153.4450914E-617.608414
CCGGCGC800.00170924616.87595722
TCTATAC1501.4205216E-716.4997333
TGCTTGA6550.016.48828345
GCGTTAT850.002561532715.88441243
CCGCCGG850.00256527515.88093719
GTGAACG850.00256527515.8809379
ATACTGA2151.5279511E-1015.6974216
TATACTG2353.6379788E-1115.3189015
ACACCAT2850.014.9997586
Produced by FastQC (version 0.11.5)