FastQCFastQC Report
Thu 26 May 2016
SRR2094113_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094113_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464395
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA35930.7736948072223No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT35460.7635741125550447No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT22070.4752419815028155No Hit
ACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCA12490.2689520774340809No Hit
TATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGT12050.25947738455409725No Hit
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAAC11460.24677268273775557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11090.2388053273614057No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA9910.21339592372872232No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA9500.20456723263601032No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT9190.19789188083420367No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA8300.17872716114514584No Hit
GGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGC8290.1785118272160553No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT8030.17291314505970135No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG7900.17011380398152437No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6860.14771907535610848No Hit
GTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGG6320.13609104318521947No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5260.11326564670162255No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC5250.11305031277253201No Hit
ACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCA5080.10938963597799288No Hit
CATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAA4980.10723629668708749No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC4880.10508295739618213No Hit
GCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGT4790.1031449520343673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4730.10185294845982407No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGA4700.10120694667255245No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGCC300.00514714629.99641211
GCGGCGA500.0022627522.49973129
GCGACGA550.00393333120.45430232
ACCTGGG2200.018.408873
GCTTACC11800.018.1141927
AGGATGT750.00110815718.0017226
TTACCAT12100.017.6650829
TAGAGCT1551.0837539E-817.4210224
CTTACCA12150.017.40532528
CTCGGGC800.001708909416.8747983
TACCATG12700.016.83050730
GATACCA18800.016.15498218
TACCTGG2650.016.1318842
CTGCTTA13900.016.02498825
TGCTTAC13850.015.92038726
ATACTCC850.00256044215.8838734
GGGCCGA850.00256044215.8838736
CCGACGG850.00256412515.88045219
ATACCAC19150.015.8597219
TGATACC19300.015.7364617
Produced by FastQC (version 0.11.5)