FastQCFastQC Report
Thu 26 May 2016
SRR2094112_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094112_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2523476
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA164780.6529881797964395No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT154220.6111411402367212No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT70650.2799709606907298No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA55590.22029137586408587No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT49600.19655427671988954No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC48110.19064972284261866RNA PCR Primer, Index 46 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC43870.1738475024133378No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43250.17139057395433918No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43070.17067727214366216No Hit
GGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGC42240.16738815823887368No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC42070.1667144843065676No Hit
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAAC39360.1559753292680414No Hit
TATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGT38910.15419207474134883No Hit
ACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCA34950.13849943490645442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31760.1258581417061228No Hit
GTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGG31730.12573925807100997No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG31200.12363898051734987RNA PCR Primer, Index 46 (95% over 24bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT28270.11202801215466286No Hit
CTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCG28260.1119883842762919No Hit
GCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTT27120.10747080614200413No Hit
ACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGC26050.10323062315631296No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA25340.10041704379197584RNA PCR Primer, Index 46 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA751.6992562E-623.9995659
CGCGTAA802.977562E-622.49959410
ACGCCCG750.001110858317.99967421
GGGTCGC1305.7880425E-717.3080656
GCACCGT1459.678661E-817.0693326
TGGACCG1205.1769803E-616.8753625
TACCGTA800.001711363816.8753627
GCGTAAC1257.636159E-616.19938711
TGTACCG3100.014.5164415
GTATACG1108.5572223E-414.3261561
CGTCTTC18750.014.27974245
CCGTCTT18800.014.12208644
GTGCGCC2408.058123E-1014.06196711
CGTGCGC2102.994966E-813.92832110
CGCCTTA1301.9272127E-413.84645233
CGTAGGT1000.007554213513.5000253
GCGTGCG2501.4861143E-913.4997569
CGGCGTT1504.275993E-513.49948941
GCCGTCT19800.013.40858443
ATACCGT1851.5293463E-613.3786676
Produced by FastQC (version 0.11.5)