FastQCFastQC Report
Thu 26 May 2016
SRR2094111_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094111_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1484930
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA36330.2446579973466763No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC32380.21805741684793223TruSeq Adapter, Index 3 (95% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT31390.21139043591280396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30640.20633969278013106No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT29160.19637289299832317No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC28230.19010997151380873No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23270.15670772359639848No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA22310.15024277238657716No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA21140.14236361309960738TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG17360.11690786771093586RNA PCR Primer, Index 3 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16870.11360804886425623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGG500.00226565822.4987375
ACGCTAC1351.9663275E-919.99887817
CGAAATC706.9874217E-419.28463413
CGCTACG1403.092282E-919.28463418
CGAACGT750.001109845818.00141345
CGTATTA750.001110598417.99959628
CCCCGTA750.001110598417.99959625
CGCTATC1507.2741386E-917.9989923
CCCGTAT800.001711419816.8746226
GACGCTA1601.6156264E-816.87405416
ACGTAGG1803.912646E-916.24963627
TAACCGT850.00256486915.88306733
CGGACTG900.003752646715.00117937
CCGACCA1351.5830085E-514.9991599
CGTAGGC1705.8924707E-714.55849728
ACGCTAT2052.1806045E-814.26749122
CCGGACT950.005374188514.21164336
TACCGTC950.005378992814.2097297
TCCGACC1605.0314575E-614.0617118
GAGACGC2102.993147E-813.9277914
Produced by FastQC (version 0.11.5)