FastQCFastQC Report
Thu 26 May 2016
SRR2094110_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094110_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297343
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA91350.3976332659076159No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT64370.28019324933194567No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA57380.24976679581586203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40490.17624708195511074No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA39400.17150247046261702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36710.15979329164169215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31240.13598317708761817No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT31140.13554789162959122No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30950.13472084925934003No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA25010.1088648930525394No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT24560.10690610849141813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG1202.5014378E-718.74852218
TACCGGC1002.050239E-517.9977977
CGCGGTT800.001712949116.87293610
CCGATCG900.003757411614.99881718
ACCGTGC2405.0931703E-1114.9981658
CGTGCGC1402.2344617E-514.46251610
GCGTCGT950.005378657514.21033527
GACGCTA1758.186071E-714.14174216
ATCTCCG8850.013.72713410
ATACGGG1150.001203529113.6939766
CGATCGC1000.007557973713.49893619
TGTACGG1000.007560149813.4983485
ACGCTAC1851.5309015E-613.37732417
CGCGTCC1352.655523E-413.33228118
TAGACTC3900.013.2676075
TACCTGG14450.013.2336752
AATCTCG8850.013.22188836
CGCCCGA2053.3682045E-713.17055326
TCGCGTC1200.001658268213.12396517
CGAGACG2401.2156306E-813.12339513
Produced by FastQC (version 0.11.5)