FastQCFastQC Report
Thu 26 May 2016
SRR2094109_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094109_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418906
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC42760.30135893427753496TruSeq Adapter, Index 10 (95% over 22bp)
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA35050.24702129668913936No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC33910.23898693782392913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29350.2068495023630882No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT28710.2023389851054263No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA28300.19944943498723666No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT27980.19719417635840572No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG26250.18500168439628842RNA PCR Primer, Index 10 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24600.17337300709137884No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA24100.1698491654838305No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA20720.14602799621680365TruSeq Adapter, Index 10 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19050.13425836524759216No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG600.00651258718.74932516
GGACCGA600.00651258718.7493256
ATCTCGC851.2047007E-418.52939843
GCTCGTA851.2047007E-418.52939826
CTCGCTA750.001110403717.99998745
GCGACGA750.001110666317.99935239
TCGCACG800.001711121416.87498720
CTCGTAG952.8207147E-416.5783527
CGTACAC952.8207147E-416.578353
GTACCGT1004.1690402E-415.7494336
ATAAGGG2051.3187673E-915.3653013
GGCGACG900.003754518514.99998938
ATACCGT900.003755398814.999466
CCGTCTT15650.014.66452544
ATAAGAC3600.014.3744823
CTCCGAC1108.5901434E-414.31817121
TCCGGTG1108.592435E-414.31766612
CGTCTTC15950.014.24763845
TGCTTGA8250.014.18180745
TTATCGA1301.9272172E-413.84565616
Produced by FastQC (version 0.11.5)