FastQCFastQC Report
Thu 26 May 2016
SRR2094108_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094108_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6670102
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC109770.1645701969774975RNA PCR Primer, Index 23 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89390.13401594158530108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73880.1107629238653322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60400.028.9884411
GTATCAA116150.020.7977491
ATCTCCG34900.015.7937110
TATCAAC156150.015.2291792
ATCAACG154800.015.1875913
TCAACGC155900.015.0804294
CAACGCA158150.014.8516555
AACGCAG163750.014.3849696
CTCGTAT38500.013.733253539
TCTCCGA40800.013.620111
CGAGACC39700.013.48937423
TATGCCG39200.013.37492943
ACGAGAC40950.013.351850522
CGAGCCC41300.013.23730915
TGCCGTC39950.013.237082545
TCTCGTA39050.013.1941338
CACGAGA40500.013.16686621
TCGTATG40200.013.15269240
CCACGAG40650.013.00660220
ATGCCGT40500.013.00175744
Produced by FastQC (version 0.11.5)