FastQCFastQC Report
Thu 26 May 2016
SRR2094107_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094107_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722223
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC44760.619753178727346TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG25630.35487654090218673RNA PCR Primer, Index 1 (95% over 24bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA24890.3446303980903405TruSeq Adapter, Index 1 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17330.23995358774229011No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14140.19578440453987203No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTT13070.1809690358794998TruSeq Adapter, Index 1 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC12580.17418442780138546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10330.14303061519779903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6600.028.654131
GAATACG507.151558E-527.00178523
TGCTTGA6050.024.54707745
CGTAGGC551.3675436E-424.5453828
GTATCAA13550.022.5970361
ATACGTA702.6983713E-522.50304825
ACGTAGG602.464017E-422.50304627
GCATGCG500.002264753222.4983719
CTGCTTG7350.020.81770344
TATCAAC25950.020.4751221
ATCAACG25950.020.4609492
TCAACGC27000.019.7485713
CAACGCA27600.019.3192544
GCGCCAC600.006510923618.74864613
AACGCAG29050.018.4324025
TACGTAG750.001108671918.00243826
GATATAG1802.055458E-1017.5108571
TCTGCTT9450.016.90470943
AATACGT850.002561611115.88450424
GGCGTGC850.002565164115.8812058
Produced by FastQC (version 0.11.5)